{"id":529,"date":"2018-04-19T20:39:19","date_gmt":"2018-04-19T20:39:19","guid":{"rendered":"http:\/\/haema-journal.gr\/?p=529"},"modified":"2018-04-25T18:31:37","modified_gmt":"2018-04-25T18:31:37","slug":"guidelines-for-the-molecular-monitoring-of-patients-with-cml-treated-with-tyrosine-kinase-inhibitors","status":"publish","type":"post","link":"https:\/\/haema-journal.gr\/?p=529","title":{"rendered":"Guidelines for the molecular monitoring of patients with CML treated with tyrosine kinase inhibitors"},"content":{"rendered":"<p style=\"text-align: right;\"><em>Haema 2012; 3(1): 57-64<br \/>\n<\/em><\/p>\n<p>by Tassoula Touloumenidou, Apostolia Papalexandri, Kostas Stamatopoulos<\/p>\n<p>Hematology Department and HCT Unit, G. Papanicolaou Hospital, Thessaloniki, Greece<\/p>\n<p style=\"text-align: right;\"><a href=\"http:\/\/haema-journal.gr\/PDF\/2012\/HAEMA_2012-57.pdf\" target=\"_blank\" rel=\"noopener\">Full PDF (in Greek)<\/a> | <a href=\"http:\/\/haema-journal.gr\/PDF\/2012\/HAEMA_2012-57.pdf\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-390 size-full\" src=\"http:\/\/haema-journal.gr\/wp-content\/uploads\/2018\/02\/PDF-icon.jpg\" alt=\"\" width=\"36\" height=\"36\" \/><\/a> <\/p>\n<p><!--more--><\/p>\n<h5><strong>Abstract<\/strong><\/h5>\n<p style=\"text-align: justify;\">The advent of therapies with a curative potential for patients with hematological malig- nancies created the need for better assessment of response. Hence, sensitive and accurate detection of minimal residual disease (MRD) is considered as a pre-requisite for therapeutic success. MRD assessment in chronic myeloid leukemia (CML) is based on the detection of the chimeric <em>BCR-ABL <\/em>mRNA transcripts which serve as a \u201cmolecular signature\u201d which can be used for the discrimination of residual malignant cells from normal hematopoietic cells. Modern PCR protocols for the detection of <em>BCR- ABL <\/em>transcripts fulfil some important criteria: in particular, (i) they provide specificity for malignancy and, (ii) they are characterized by satisfactory sensitivity. However, limitations persist: thus, standardization is suboptimal or not fully implemented, while the precise quantification of MRD levels is still an open issue. Today, the molecular monitoring of <em>BCR-ABL <\/em>transcripts in CML is performed by real-time quantitative PCR (RQ-PCR) protocols. Thanks to international multi-institutional initiatives, recommendations for harmonizing the differing RQ-PCR methodologies with the objective of maximizing reliability of analysis for clinical decision making now exist: all aspects of the analysis are covered, from source and number of cells to validation of PCR amplification efficiencies, ensuring the comparability of the results from different trials and\/or medical centers. A cornerstone of the follow-up of CML patients treated with tyrosine kinase inhibitors relates to the accurate detection of somatic mutations with- in the <em>BCR-ABL <\/em>kinase domain, which represent the major mechanism of acquired resistance. Several approaches are available for mutation detection; however, presently the most widely used method is direct sequencing. More sensitive approaches may be beneficial, but are yet to prove their clinical utility.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Haema 2012; 3(1): 57-64 by Tassoula Touloumenidou, Apostolia Papalexandri, Kostas Stamatopoulos Hematology Department and HCT Unit, G. Papanicolaou Hospital, Thessaloniki, Greece Full PDF (in Greek) |<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[41],"tags":[],"class_list":["post-529","post","type-post","status-publish","format-standard","hentry","category-haema-2012-1"],"_links":{"self":[{"href":"https:\/\/haema-journal.gr\/index.php?rest_route=\/wp\/v2\/posts\/529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/haema-journal.gr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/haema-journal.gr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/haema-journal.gr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/haema-journal.gr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=529"}],"version-history":[{"count":4,"href":"https:\/\/haema-journal.gr\/index.php?rest_route=\/wp\/v2\/posts\/529\/revisions"}],"predecessor-version":[{"id":984,"href":"https:\/\/haema-journal.gr\/index.php?rest_route=\/wp\/v2\/posts\/529\/revisions\/984"}],"wp:attachment":[{"href":"https:\/\/haema-journal.gr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=529"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/haema-journal.gr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=529"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/haema-journal.gr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}